makefile - 命令在第一个目标之前开始

Question

启动makefile时收到此消息，最可能的错误是什么？它在每行的开头以制表符分隔。

makefile_first30samples_rfam:63: *** commands commence before first target.  Stop.

robinm@havpryd:~/Oluf/Project_GCF-2014-313$ head makefile_first30samples_rfam
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_007_GAGTGG.fastq > Cut_merged_007_GAGTGG.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_026_ATGTCA.fastq > Cut_merged_026_ATGTCA.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_030_GTGGCC.fastq > Cut_merged_030_GTGGCC.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_040_CACCGG.fastq > Cut_merged_040_CACCGG.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_086_AGTTCC.fastq > Cut_merged_086_AGTTCC.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_087_CCGTCC.fastq > Cut_merged_087_CCGTCC.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_125_CTTGTA.fastq > Cut_merged_125_CTTGTA.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_197_GCCAAT.fastq > Cut_merged_197_GCCAAT.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_208_CGTACG.fastq > Cut_merged_208_CGTACG.fastq
    #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_226_CAACTA.fastq > Cut_merged_226_CAACTA.fastq
robinm@havpryd:~/Oluf/Project_GCF-2014-313$ 

Answer 1

Makefile规则必须具有

形式

target: ...
        command
        command

看起来你的目标线丢失了。

尝试类似

的内容

foo:
        #cutadapt -a TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG merged_007_GAGTGG.fastq > Cut_merged_007_GAGTGG.fastq
        ...

（foo之前没有空格：每个命令前有一个标签）